RNA-Seq identified 111 DEGs, i.e., 50 down- and 61 up-regulated, that distinguished cth+ from cth− T-ALL cases (Supplementary Fig. 2a, Supplementary Table 12), and confirmed the downregulation of RGCC. Functional analysis showed the downregulation of nitric oxide related genes (i.e., AOX1 and HBA1) and the upregulation of the NOTCH signaling (including MFAP2, JAG2 and DLK1 genes), as the top enriched pathways in cth+ T-ALL (FDR < 0.1) (Supplementary Fig. 2b). Here, MFAP2 is linked to acute lymphoblastic leukemia.