We selected the best-performing CD73 antibody clone (see Methods) and performed 36-marker tissue-based cyclic immunofluorescence (CyCIF)9 on 194 glioblastoma cases, including 139 adult glioblastoma specimens for which clinical and/or genetic data were available (Fig. 7A and Fig. S11) as well as an additional set of 14 oligodendroglioma and 15 anaplastic oligodendrogliomas. This evidence concerns the gene NT5E and glioblastoma.