Most H3K27M-mutant diffuse midline gliomas exhibited high CD73 expression (IHC ≥ 2), though CD73 levels (as measured by IHC or bulk mRNA sequencing) were not significantly different than non-H3F3A mutated pediatric HGG, which also showed strong expression (Fig. S8f, g). This evidence concerns the gene NT5E and diffuse midline glioma.