Pouwels et al. [30] systematically exposed mice to CS after the intraperitoneal injection of Nec-1 and found that Nec-1 pre-treatment remarkably inhibited CS-induced neutrophilic airway inflammation by assessing the number of neutrophils, the degree of activation and inflammatory markers in the bronchoalveolar lavage fluid of mice, confirming that necroptosis has a role in airway inflammation. Here, PCSK1 is linked to inflammation.