As shown in Fig 5 and Table 3, statistical parameters determined (absolute count, proportion, intensity and overall scores) indicated significant increase (p<0.05) in the number of CD4+ (T helper cells), CD8+ (cytotoxic T cells) and IL-2+ (activated T cells) in mammary tumor tissues of TM-F3 when compared to the TM group. Here, CD8A is linked to breast cancer.