Before the in vivo experiments, mouse primary trophoblast cells (MTCs) from C57BL/6N mice at embryonic day 9.5 (E9.5) of pregnancy were isolated and cultured to investigate whether ZIKV infection could also induce the GSDME-mediated pyroptosis in mouse trophoblast cells (Figure 5—figure supplement 1A). This evidence concerns the gene GSDME and Zika virus infectious disease.