However, we noticed that infected SH-SY5Y cells did not undergo pyroptosis despite expressing a high level of GSDME, while Huh-7 and A549 cells expressing the relatively lower GSDME presented the different degrees of pyroptosis and GSDME activation upon infection (Figure 2A-C, and Figure 2—figure supplement 1A), suggesting that other factors are involved in ZIKV-induced pyroptosis. The gene discussed is GSDME; the disease is infection.