Consistent with our previous observations in JEG-3 cells, the upregulation of RIG-I caused by ZIKV infection was also seen in MTC, and inhibition of either caspase-3 or caspase-8 prominently blocked ZIKV-induced activation of GSDME (Figure 5—figure supplement 1C). Here, CASP8 is linked to Zika virus infectious disease.