ORMDL3 and hepatocellular carcinoma: PI/Hoechst double-staining kit was used to detect the effect of ORMDL3 on the apoptosis and necrosis of HCC cells, and it showed that after sorafenib (4 μM) treatment, ORMDL3 silencing increased the apoptosis and necrosis of HCC cells, while the overexpressed ORMDL3 showed the opposite effect (Fig. 2D, E).