For further research, MitoSOX Red Mitochondrial Superoxide Indicator was used to detect the mitochondrial superoxide level of ORMDL3-silenced hepatoma cells, and showed that mitochondrial superoxide levels were elevated in the presence of sorafenib compared with controls, and silencing of ORMDL3 resulted in higher levels of mitochondrial superoxide in hepatoma cells (Fig. S1 C, D). This evidence concerns the gene ORMDL3 and hepatocellular carcinoma.