They found that Gsdmd−/− mice fed on a high-fat diet (HFD) showed higher serum levels of inflammation cytokines (TNF-α, monocyte chemoattractant protein 1 [MCP-1], and IL-6), lower levels of TJ proteins (ZO-1, occludin, and claudin), and significantly higher endotoxemia than the corresponding control WT mice. This evidence concerns the gene TNF and serum lipopolysaccharide activity.