In addition, RIP assay results showed LIPH-4 and miR-216b were significantly enriched in AGO2-containing micro-ribonucleoprotein complexes, suggesting that the AGO2 protein bound directly to LIPH-4 and miR-216b in ESCC cells (Fig. 4D). This evidence concerns the gene AGO2 and esophageal squamous cell carcinoma.