DARS1-AS1 and breast cancer: Moreover, we found that the knockdown of DARS-AS1 by these shRNAs significantly inhibit cell growth of colorectal cancer cell lines SW620 (by 49.7%) and HCT116 (by 27.7%), breast cancer cell line MBA-MD-231 (by 53.4%) and liver cancer cell line HepG2 (by 92.7%), as well as their ability of unanchored sphere formation (reduced about 50.8%, 44.6%, 40.7% and 75.7% on average for each cell line) (Fig. 2a, b).