To determine if a correlation exists between βlig, efficacies and/or potencies and the size of EphA2 oligomers, we compared these EphA2 signaling parameters previously measured in PC3 prostate cancer cells (which have high expression of endogenous EphA2 (40)) with brightness distributions measured by FIF in HEK293T cells (in which we transiently expressed EphA2 labeled with eYFP). Here, EPHA2 is linked to prostate cancer.