Combinations of PGM and DNA were made with PGM at 5 mg/mL and mucin to DNA ratios of 100:1 (i.e., 0.05 mg/mL DNA) and 20:1 (i.e., 0.25 mg/mL) to mimic mucin to DNA ratios observed in CF in preschool aged children and adults, respectively (17). This evidence concerns the gene MUC5AC and cystic fibrosis.