COL3A1 and metabolic dysfunction-associated steatotic liver disease: Additionally, there was a main effect (p = .0003) of Obese to have increased gene expression of liver collagen, col3a1 (Figure 3e), and a main effect (p < .0001) of Obese to have increased total liver lipid content (Figure 3f) irrespective of 5FU treatment, further demonstrating high fat diet-induced NAFLD.