TNF and obesity due to melanocortin 4 receptor deficiency: As expected, there were main effects of Obese to have increased macrophage genes CD68 (p < .0001), EMR1 (p < .0001), Itgax (p < .0001), and MRC1 (p < .0001) (Figure 8a) and increased cytokines TNFα (p < .0001), IFNγ (p = .013), and IL-10 (p < .0001) (Figure 8b); however, the inflammatory status of adipose tissue with obesity did not appear to be impacted by 5FU (i.e. there was no main effect of 5FU).