The zoom-in images from a cancer nest area (marked as a white dashed line, and two red/green circles - IL34 and CSF1R in a red box and THY1 and ITGAM in a green box) show distinct coexpression of neighbouring cells at single-cell resolution, suggesting cell to cell interaction for each of pair compared to no signal in the ‘Negative’ control. Here, IL34 is linked to cancer.