Furthermore, topo I-reactive B cells from topo I-induced SSc model mice produced inflammatory suppression cytokines such as IL-10 and IL-35 when the affinity of BCR for topo I antigen was low, while they produced inflammatory cytokines such as IL-6 and IL-23 when the antigen affinity for topo I antigen increased with the frequency of topo I antigen-induced autoimmune triggers (71). This evidence concerns the gene IL6 and systemic sclerosis.