To visualize the alterations in the KCs morphology in a liver-specific niche during ALI, we used intravital microscopy of the liver in mice previously injected i.v. with fluorescent monoclonal antibodies (mAb) targeting the macrophage surface marker F4/80, as well as necrotic cells (DNA labeling by Sytox green) and liver vasculature using anti-CD31. The gene discussed is PECAM1; the disease is acute respiratory distress syndrome.