A distinctive differentiation pattern of FoxP3+ CD8 T-cells was observed in untreated HIV infection, characterized by an increase in CM, EM, and TD FoxP3+ CD8 T-cells that, excepting for EM FoxP3+ CD8 T-cells, were normalized by early ART, indicating an increased differentiation of FoxP3+ CD8 T-cells in HIV infection. This evidence concerns the gene CD8A and HIV infectious disease.