H&E staining of the excised tumors from mice fed with high glutamine diet showed areas of residual normal glandular parenchyma (Figure S6). IHC staining revealed that infiltrating cytotoxic CD8+ T cells were more notably abundant with overall less Foxp3+ Tregs detected in tumor sections from high glutamine-fed mice (Figure 6D, upper 4 panels). This evidence concerns the gene CD8A and neoplasm.