To further explore the role of sclerostin and its loop3 in cardiovascular system for OI in vivo, full-length human sclerostin knock-in mice (hSOSTki) and loop3 deficient human sclerostin knock-in mice (Δloop3-hSOSTki) were generated, followed by construction of hSOSTki.Col1a2+/G610C.ApoE-/- mouse model and Δloop3-hSOSTki.Col1a2+/G610C.ApoE-/- mouse model, respectively (Figure S1). Here, APOE is linked to osteogenesis imperfecta.