Previous studies have presented that AKIP1 modulates PI3K/AKT and β-catenin pathways to regulate tumor progression (12, 19, 20); thus, the effect of AKIP1 knockdown on these pathways in ATC cell lines was explored by Western blot, which revealed that AKIP1 knockdown inhibited the phosphorylation of PI3K and AKT in 8505C and CAL-62 cells (both p < 0.05); meanwhile, AKIP1 knockdown inhibited expression of β-catenin in CAL-62 cells (p < 0.05) but not in 8505C cells (p > 0.05) (Figures 3A–C). This evidence concerns the gene AKIP1 and neoplasm.