RT-qPCR for quantification of IGF1 expression in human normal ovarian cell line IOSE80 and ovarian cancer cell lines (CAOV4, CoC1 and SKOV3) presented that IGF1 expression was elevated in CAOV4, CoC1 and SKOV3 cells when compared with that in IOSE80 cell line, among which the highest IGF1 expression was detected in CAOV4 cell line and the lowest IGF1 expression was detected in SKOV3 cell line (Fig. 2A). This evidence concerns the gene IGF1 and ovarian cancer.