To validate the reliability of the RNA sequencing data, we selected 21 DEGs (cacnb1sa, acctc1c, cacnb2, cu1633479, symhc3, slc8a1a, myh7, atp2, tnn2d, ryr2b, desma, itgb1b2, lama2, tnnc1b, tnn2c, tnnc1a, myh7l, myl10, tnnc2c, symhc2, ngs), which were mainly related to cardiovascular development and cardiovascular disease pathways. This evidence concerns the gene CACNB2 and cardiovascular disorder.