Both larval ALS zebrafish models were treated as detailed above, with either PGK1 overexpression at the single cell stage or treatment with 50 mM terazosin from 6 hpf, and allowed to develop until 72 hpf (n = 12-20 larvae across 2 clutches per treatment group). Here, PGK1 is linked to amyotrophic lateral sclerosis.