In another NSCLC cohort (GSE135222), patients who benefited from immunotherapy had higher IPTSes than those who did not benefit from immunotherapy (P = 0.0451) (Fig. 5B) with a ROC AUC of 0.77 (95% CI = 0.96–0.58), and it was also larger than that of PD-L1 of 0.69 (95% CI = 0.90–0.48, Fig. 5E), suggesting that the IPTS has a good predictive value of immunotherapy efficacy in this cohort. The gene discussed is CD274; the disease is non-small cell lung carcinoma.