Here, we report that although melatonin does not change glycaemic levels, consistent with other report [24], it mitigates the impairment in steroidogenesis caused by hyperglycaemia in rat testes through activation of SIRT1 pathway, resulting in enhanced mitochondrial function, autophagy and mitophagy activity as well as mitigated oxidative stress. Here, SIRT1 is linked to Hyperglycemia.