Thus, we evaluated whether TAMs can induce EMT in ESCC cells and found that KYSE410 and KYSE510 cells cocultured with pol-TAMs, pri-TAMs or CCL22 (50 ng/ml) underwent a transition from an epithelial-like phenotype to an elongated phenotype, with a decrease in E-cadherin expression and increases in vimentin and N-cadherin expression (Fig. 3C, D). This evidence concerns the gene CDH2 and esophageal squamous cell carcinoma.