These data suggested that PBX1 rescues SIRT1-knockdown-mediated HF-MSCs senescence and apoptosis by alleviating ROS-mediated DNA damage and intracellular NAD depletion and that the SIRT1–PARP1 axis plays a critical role in PBX1-alleviated HF-MSCs senescence and apoptosis. Here, SIRT1 is linked to hydrops fetalis.