All sgBcl-2 lymphoma-derived cell lines displayed higher expression of BCL-2 and the pro-apoptotic BH3-only protein BIM, compared with the control cell lines derived from lymphomas containing non-targeting control sgRNAs (#219, #220), as detected by both Western blotting (Fig. 5a) and intracellular flow cytometric analysis (Fig. 5b). The gene discussed is BCL2L11; the disease is lymphoma.