To further explore the role of miR-596-3p and YAP1 on NSCLC brain metastasis, we detected the transendothelial cell migration capacity of brain metastasis cells by using an in vitro blood-brain barrier (BBB) model, which was established by employing the transwell chamber coated with human brain endothelial cells (HBMEC) and normal human astrocytes (NHA) (Fig. 5A). This evidence concerns the gene YAP1 and non-small cell lung carcinoma.