The mixed polarization of M1/M2 type in THP-1 treated with tumor-soluble factors was also observed in colorectal cancer, in which CM from several colon cancer cell lines enhanced the phagocytic activity of THP-1 cells [25] and induced the production of cytokines and chemokines typical of both M1 and M2 macrophages, including IL-1β, MCP-1, IL-6, and IL-10 [37]. Here, CCL2 is linked to colorectal cancer.