In the study, mimics miR-22 could remarkably downregulate the LPS-induced increased TLR4, TLR2, MyD88, and p-NF-κB p65 protein expression, which indicated that miR-22 may alleviate sepsis-induced AKI by targeting HMGB1/TLR4/NF-κB signaling pathway. Here, NFKB1 is linked to acute kidney injury.