Hence, in this study, we induced an animal model of sepsis-related AKI by cecal ligation and puncture (CLP) method and used LPS to construct the AKI cell model to evaluate the role of miR22 and HMGB1 in sepsis-related AKI, providing a scientific basis for further development of miR-22 as a novel therapeutic target for treating AKI. This evidence concerns the gene HMGB1 and acute kidney injury.