NPPB and familial dilated cardiomyopathy: Differential expression analysis by pseudobulk and single-cell approaches across disease state revealed a large number of genes significantly upregulated (NPPA, NPPB, ACE2 and KIF13A) and downregulated (MYH6, ADRB2 and CKM) in DCM samples compared to non-diseased donors (Fig. 3b).