Differential expression analysis by pseudobulk and single-cell approaches across disease state revealed a large number of genes significantly upregulated (CCL3, NLRP3, NFKB2 and EGR1) and downregulated (VSIG4, LYVE1, FMN1 and CD163) in DCM samples compared to non-diseased donors (Fig. 4b). This evidence concerns the gene NLRP3 and familial dilated cardiomyopathy.