The Transwell invasion assay was performed to compare the invasion (Figure 5, Table 2) and migration (Figure 6, Table 3) ability of A549 lung adenocarcinoma cells in the blank control group, the Tim-3 blockade group, the PD-1 blockade group, and the Tim-3+PD-1 blockade group. This evidence concerns the gene HAVCR2 and lung adenocarcinoma.