In addition, we developed methods to evaluate the degree of IgE-mediated mast cell degranulation in oral cavities, reflecting the severity of OAS in murine model: immediately after an injection of OVA inside the lower lip in mice with high levels of serum OVA-specific IgE, an intravenous injection of the Evans blue dye induced apparent dye extravasation in the neck skin, of which amounts could be quantitated. The gene discussed is IGHE; the disease is microphthalmia with limb anomalies.