Further, collagen type I alpha 1 chain (COL1A1) (14) and thrombospondin 2 (TSP2) (15) exhibited the highest expression levels in molecular subtype I as biomarkers of the advanced fibrosis stage of NAFLD (Figures 8A, B), demonstrating that molecular subtype I had the most significant fibrosis. The gene discussed is THBS2; the disease is metabolic dysfunction-associated steatotic liver disease.