To further determine the effect of RLRs/TLRs signaling on PEDV-induced ISGs production, LLC-PK1 cells were transfected with specific siRNAs targeting adaptor molecules in the RLRs and TLRs signaling pathways such as RIG-I, MDA5, MAVS, MyD88 and TRIF, followed by FJzz1 infection, and the expression of various ISGs was analyzed by RT-qPCR. The gene discussed is IFIH1; the disease is infection.