Furthermore, by using a transwell system of tumor cells co-cultured with the PMA-differentiated human THP-1 monocytes (M0 macrophages), they found CMTM6 in tumor cells was negatively correlated with M1-specific markers CD80 and CD86, while positively correlated with M2-specific marker CD163 in macrophages, further supporting the conclusion that CMTM6 promotes M2 polarization. This evidence concerns the gene CD86 and neoplasm.