GNAS and ischemia: On laser doppler perfusion imaging of distal ischemia foot or normal foot, blood perfusion of distal limb was significantly impaired in GsαECKO mice at days 3, 7, or 14 after ischemic injury as compared with CTR mice (Figures 2A,B), which showed that endothelial Gsα deficiency impaired the post-ischemia angiogenesis.