In addition, depletion of the PHOSPHO1 gene ameliorated HFD-induced obesity, MAFLD, and insulin resistance in mice, indicating that PHOSPHO1 negatively regulates BAT activation and energy metabolism (Gliniak and Scherer, 2020; Jiang et al., 2020). The gene discussed is PHOSPHO1; the disease is obesity due to melanocortin 4 receptor deficiency.