SEMA3D and hepatocellular carcinoma: We found that Sema3d restrained HCC growth and metastasis in vitro and in vivo. To elucidate the mechanism and screen the potential signaling manipulated by Sema3d, RNA-seq was utilized to compare the expression profiles between HCCLM3Ctr and HCCLM3Sema3d cells and demonstrated that 75 mRNAs upregulated and 282 mRNAs downregulated at least 2-fold change in HCCLM3Sema3d cells (Supplementary Figure S5, the data uploaded at the GEO database: GSE200430).