In the training and validation cohorts, a low expression level of Sema3d (IHC score ≤4) was closely correlated with tumor size, tumor nodular number, micro- and macrovascular invasion, advanced tumor node metastasis stage (TNM), Barcelona Clinic Liver Cancer (BCLC) stage, and China Clinic Liver Cancer (CNLC) stage (25) (all p < 0.05, Supplementary Table S1). This evidence concerns the gene SEMA3D and liver cancer.