In addition, we used western blot and immunohistochemistry to assess Notch1, Jagged1 and Hes1 expression in tumor tissues from nude mice, and the results demonstrated a reduced expression of Notch/Jagged1/Hes1 signaling pathway in tumor tissues from the shCEMIP group in comparison to the control group (Figures 6D–G). This evidence concerns the gene HES1 and neoplasm.