To further investigate the role of CEMIP in pathogenesis of osteosarcoma in vitro, CEMIP expression was determined using qPCR and western blot in a panel of osteosarcoma, and the results indicated that it was most abundantly expressed in HOS and U2OS (Figures 2A–C), which were used in the subsequent assays. The gene discussed is CEMIP; the disease is osteosarcoma.