Through WES and filtering against several publicly accessible variation databases and eliminating all previously reported variants, other glaucoma-associated genes, including MYOC, CYP1B1, FOXC1, LRP2, PITX2, PAX6, LTBP2, TEK, ANGPT1, and PMEL, were excluded in these two pedigrees. The gene discussed is LTBP2; the disease is glaucoma.