In this study, we aimed to determine the function of NCKAP1 in ccRCC using bioinformatics analysis portal tools and immunohistochemical validation, to examine the relationship between NCKAP1 expression and clinicopathological features of ccRCC, and to determine the in vitro and in vivo NCKAP1 expression was measured to characterize the clinicopathological features of ccRCC. Here, NCKAP1 is linked to nonpapillary renal cell carcinoma.