In this regard, the findings of this study revealed that tRF‐24‐V29K9UV3IU which was downregulated in GC, can target several genes connected with traditional signalling pathways that are implicated in tumour occurrence and metastasis, including MAPK (MAPK8IP1, MAPK8IP2, MAPK8IP3, MAP2K3, MAP3K7, etc.), chemokine (CX3CL1, CX3CR1, CXCR3, CXCR5, CXCL9, etc.), Wnt/β‐Catenin (WNT4, WNT10A, WNT3A, etc.), and ErbB (ErbB2) signalling pathways. Here, EGFR is linked to gastric cancer.