ESR1 and ocular melanoma: In addition, UF-treated cells showed a significant increase in β-catenin protein levels (semi-quantified by immunofluorescence analysis, p < 0.05 vs. OM), which were significantly reduced following 24 and 72 h tamoxifen treatment (p < 0.05 vs. OM + UF), suggesting that the role exerted by UF in cultured hOBs can be mediated by the ESR1/β-catenin pathway (Figure 5B).