Although macrophage infiltration in adipose tissue of these mice was observed after this short-term intervention, adipose tissue macrophages appeared not to be essential for the development of glucose intolerance or insulin resistance, as mice with a clodronate-mediated >80% depletion of macrophages still became glucose intolerant and insulin resistant after three days of consuming a high-fat diet [158]. This evidence concerns the gene INS and Insulin resistance.