A seven day high-fat diet feeding increased adipocyte plasma membrane 1,2-diacylglycerol content, activated PKCε and impaired insulin-dependent suppression of lipolysis supposedly by phosphorylation of Thr1150 in the insulin receptor β-chain, as mice with a Thr1150Ala substitution in the receptor were protected from insulin resistance [164]. Here, INSR is linked to Insulin resistance.