Genomic DNA was extracted from the blood and tumour tissues of all patients using the phenol chloroform method and DNA extraction kit (Zymo Research Corporation, Irvine, CA, USA). PCR was conducted using DNTP (Sigma-Aldrich, St. Louis, MO, USA), 10 mMdTTP primers (Sigma-Aldrich, St. Louis, MO, USA) and Taq DNA polymerase (Biotools, Madrid, Spain). PCR-RFLP was performed using a restriction endonuclease enzyme (New England Biolabs, NEB, England). APOA1 concentrations were measured using standard source ELISA kit (Thermo Scientific, Waltham, MA, USA). This evidence concerns the gene APOA1 and neoplasm.