To clinically investigate whether TLR4 mRNA expression is associated with the expression LIN28 mRNA or let-7 miRNA, 20 human HCC tissues (Figure 1) were further analyzed for the expression of LIN28A and LIN28B mRNA and let-7b, let-7i and let-7g miRNA (Supplementary Figure S4). Here, LIN28B is linked to hepatocellular carcinoma.