SIRT3 increases mitochondrial fuel supplies through the deacetylation of long-chain acylcoenzyme A dehydrogenase (LCAD), which regulates fatty acid β-oxidation [36], and there is evidence that LCAD was hyperacetylated in the liver of Sirt3-deficient mice fed with HFD that had severe hepatic steatosis, consistent with the reduced fatty-acid oxidation [19]. Here, SIRT3 is linked to fatty liver disease.