To determine whether SIRT3 SUMOylation also occurs in AML, the whole cell protein lysates were extracted from vector control, SIRT3, SIRT3K288R overexpressing MV4-11 cells and immunoprecipitated with SUMO1 antibody followed by immunoblotting with SIRT3 antibody. This evidence concerns the gene SIRT3 and acute myeloid leukemia.