Firstly, Sin et al. applied RNA sequencing tool for discovering bladder-cancer specific, 3-component mRNA panel, ergo urinary concentration of ROBO1 (promoter of tumor angiogenesis, BCa specific), WNT5A (regulation of cell polarity and migration, BCa specific) and CDC42BPB (the reference gene) mutational load. The gene discussed is CDC42BPB; the disease is urinary bladder carcinoma.